IL-4 Antibody (3007) [Phycoerythrin]

Images

 
NS0 mouse myeloma cell line transfected with human IL-4 was stained with Mouse Anti-Human IL‑4 PE‑conjugated Monoclonal Antibody (Catalog # IC204P, filled histogram) or isotype control antibody (Catalog # IC002P, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICFlow
Clone
3007
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

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Catalog# & Conjugate Size Price

IL-4 Antibody (3007) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human IL‑4
His25-Ser153
Accession # P05112.1
Specificity
Detects human IL-4 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑4.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL4
Purity
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC204P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from ascites

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4 Antibody (3007) [Phycoerythrin]

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the Common gamma  Chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-4 Antibody (IC204P-100)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Chip Cytometry, Flow.


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Chip Cytometry
(1)
Flow
(3)
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Human
(4)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL4
Entrez
Uniprot