IL-38/IL-1F10 Antibody Pair

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell Culture Supernatant, Serum
• Standard Curve Range: 0.031 - 2 ng/ml
• Sensitivity: 20 pg/ml

IL-38/IL-1F10 Antibody Pair Summary

• Description: Sandwich ELISA for the quantitative determination of Human IL-38/IL-1F10
• Standard Curve Range: 0.031 - 2 ng/ml
• Sensitivity: 20 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: IL1F10

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA
• Application Notes: 1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (rabbit polyclonal antibody) to 5 ug/ml in PBS without carrier protein and use it fresh.
  2. Dilute the desired amount of Detection Antibody (DET) (1 mg/ml) (mouse monoclonal antibody) to 1 ug/ml in ELISA Buffer and use it fresh.
  3. Reconstitute the Anti-IgG-HRP (HRP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted HRP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted AntiIgG-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
  4. Reconstitute the Standard Protein (STD) (recombinant human IL-38/IL-1F10) with 100 ul ELISA Buffer to obtain a concentration of 10 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 2 ng/ml, 1 ng/ml, 0.5 ng/ml, 0.25 ng/ml, 0.125 ng/ml, 0.062 ng/ml, 0.031 ng/ml and 0 ng/ml.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for IL-38/IL-1F10 Antibody Pair

• FIL1- theta
• FKSG75
• IL1F10
• IL-1HY2
• IL38
• IL-38
• interleukin 1 family, member 10 (theta)
• UNQ6119/PRO20041

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL1F10