IL-36Ra/IL-1F5 Antibody - BSA Free

Western Blot: IL1F5 Antibody [NBP1-77873] - Shows detection of recombinant bovine IL-1F5 at 17.1 kDa (arrowhead) raised in yeast. The identity of the band at 36 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 (TM) conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.

• Reactivity: Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

IL-36Ra/IL-1F5 Antibody - BSA Free Summary

• Description: This product was Protein-A purified from monospecific antiserum by chromatography
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This protein-A purified IL-36Ra/IL-1F5 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 155 amino acids of the mature bovine IL-36Ra/IL-36Ra/IL-1F5 protein. (Uniprot: Q0VC52)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL36RN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:8000
  • Western Blot 1 ug/ml
• Application Notes: This protein-A purified This product has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17.1 kDa in size corresponding to bovine IL-1F5 protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with IL-36Ra/IL-1F5 from dog, panda, and ferret based on 90-92% homology with the immunizing sequence.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for IL-36Ra/IL-1F5 Antibody - BSA Free

• FIL1 delta
• FIL1
• FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta)
• FIL1DIL-1 delta
• IL1F5 (Canonical product IL-1F5a)
• IL1F5
• IL-1HY1
• IL-1L1
• IL-1RP3
• IL36Ra
• IL-36Ra
• IL36RN
• interleukin 1 family, member 5 (delta)
• interleukin 1, delta
• Interleukin 36 Receptor Antagonist
• interleukin-1 family member 5
• Interleukin-1 HY1
• Interleukin-1 receptor antagonist homolog 1
• Interleukin-1-like protein 1
• Interleukin-36 Receptor Antagonist

Background

The protein encoded by the IL1F5 gene is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding the same protein have been reported. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL36RN
• Uniprot:
  • Q0VC52(Human)