IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free

Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.

Immunocytochemistry/Immunofluorescence: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6.

CyTOF-ready: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IL1F6 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human IL1F6 (NP_055255) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL36A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 17.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free

• FIL1 epsilon
• FIL1
• FIL1(EPSILON)
• FIL1E
• IL-1 epsilon
• IL1(EPSILON)
• IL1E
• IL-1E
• IL-1F6 (FIL-1-epsilon)
• IL1F6
• IL-1F6
• IL36 alpha
• IL-36 alpha
• IL36A
• interleukin 1 family, member 6 (epsilon)
• interleukin 1, epsilon
• Interleukin 36, Alpha
• Interleukin-1 epsilon
• interleukin-1 family member 6
• Interleukin-36 Alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL1F6 Antibody (NBP2-71834)(1)

Publication using NBP2-71834

• Hu, M, Tong, Y Et al. IL36 indicating good prognosis in human Hepatocellular Carcinoma. J Cancer 2020-10-10 [PMID: 33033508] (WB, Human)

Bioinformatics

• Gene Symbol: IL36A