IL-36 alpha/IL-1F6 Antibody (OTI1A4)

Western Blot: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.

Immunocytochemistry/Immunofluorescence: IL1F6 Antibody (1A4) [NBP2-02758] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6.

Flow Cytometry: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IL1F6 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: OTI1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IL-36 alpha/IL-1F6 Antibody (OTI1A4) Summary

• Description: Novus Biologicals Mouse IL-36 alpha/IL-1F6 Antibody (OTI1A4) (NBP2-02758) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. Anti-IL-36 alpha/IL-1F6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human IL1F6 (NP_055255) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL36A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 17.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IL-36 alpha/IL-1F6 Antibody (OTI1A4)

• FIL1 epsilon
• FIL1
• FIL1(EPSILON)
• FIL1E
• IL-1 epsilon
• IL1(EPSILON)
• IL1E
• IL-1E
• IL-1F6 (FIL-1-epsilon)
• IL1F6
• IL-1F6
• IL36 alpha
• IL-36 alpha
• IL36A
• interleukin 1 family, member 6 (epsilon)
• interleukin 1, epsilon
• Interleukin 36, Alpha
• Interleukin-1 epsilon
• interleukin-1 family member 6
• Interleukin-36 Alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL1F6 Antibody (NBP2-02758)(1)

Publication using NBP2-02758

• Hu, M, Tong, Y Et al. IL36 indicating good prognosis in human Hepatocellular Carcinoma. J Cancer 2020-10-10 [PMID: 33033508] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: IL36A
• Entrez:
  • 27179(Human)