Human IL-34 Recombinant Protein

Product Discontinued

Human IL-34 Recombinant Protein Summary

• Description: A single glycosylated polypeptide protein with C-terminal 6-His tag corresponding to amino acids Asn21 - Pro242 of IL - 34 (NP_689669).
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Lyophilized from 0.2 mm filtered concentrated solution in PBS and NaCl.
• Details of Functionality: The ED50, as determined by a cell proliferation assay using human peripheral blood monocytes, is less than 3.75 ng/mL.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: IL34
• Purity: SDS-PAGE
• Endotoxin Note: Less than 1 EU/mg of the rHuIL-34 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with PBS to a final concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human IL-34 Recombinant Protein

• C16orf77
• IL34
• IL-34
• interleukin 34

Background

Interleukin 34 (IL-34) is a 39-45 kDa homodimeric cytokine that signals through M-CSF R/CSF1R. It is secreted by synovial fibroblasts, neurons, keratinocytes, splenic sinusoidal endothelial cells, and osteoblasts. It promotes the development and survival of osteoclasts, macrophages, Langerhans cells, and neurons. In brain, IL-34 induces TGF-B, HO-1, and IDE production by microglia and may additionally bind and signal through PTP-z.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL34