Human IL-34 Recombinant Protein Summary
| Description |
A biologically active protein with C-terminal 6-His tag corresponding to amino acids Asn21 - Pro242 of IL - 34. Source: Mouse myeloma cell line, NS0-derived |
Preparation Method |
Lyophilized from a 0.2 um filtered solution in PBS and NaCl. |
| Details of Functionality |
Measured in a cell proliferation assay using human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The ED50 for this effect is typically 0.75 - 3.75 ng/mL. |
| Source |
Nso cells |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
IL34 |
| Endotoxin Note |
<0.10 EU per 1 ug of the protein by the LAL method. |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
26.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Concentration |
LYOPH |
| Reconstitution Instructions |
Reconstitute with 0.1 mg/ml in sterilized PBS containing at least 0.1% human or BSA to desired concentration. |
Notes
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Human IL-34 Recombinant Protein
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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