IL-34 Antibody (578416) [Phycoerythrin]

NS0 mouse myeloma cell line transfected with human IL-34 was stained with Mouse Anti-Human IL-34 PE-conjugated Monoclonal Antibody (Catalog # IC5265P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 578416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-34 Antibody (578416) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-34
  Asn21-Pro242
  Accession # Q6ZMJ4
• Specificity: Detects recombinant human IL-34 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-34 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL34
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-34 Antibody (578416) [Phycoerythrin]

• C16orf77
• IL34
• IL-34
• interleukin 34

Background

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1,2). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1,3).

1. Lin, H. et al. (2008) Science 320:807.
2. Chihara, T. et al. (2010) Cell Death Differ. 17:1917.
3. Wei, S. et al. (2010) J. Leukoc. Biol. 88:495.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-34 Antibody (IC5265P)(2)

Publications using IC5265P

• E Picarda, S Bézie, L Usero, J Ossart, M Besnard, H Halim, K Echasserie, C Usal, J Rossjohn, K Bernardeau, S Gras, C Guillonnea Cross-Reactive Donor-Specific CD8+ Tregs Efficiently Prevent Transplant Rejection Cell Rep, 2019-12-24;29(13):4245-4255.e6. 2019-12-24 [PMID: 31875536] (Flow Cytometry, Human)
• Bezie S, Picarda E, Ossart J, Tesson L, Usal C, Renaudin K, Anegon I, Guillonneau C IL-34 is a Treg-specific cytokine and mediates transplant tolerance. J Clin Invest, 2015-09-21;125(10):3952-64. 2015-09-21 [PMID: 26389674] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL34
• Uniprot:
  • Q6ZMJ4(Human)