IL-33 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

IL-33 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human IL-33 Propeptide
  Met1-Ser111
  Accession # O95760
• Specificity: Detects human IL-33 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human IL-33 and mature recombinant mouse IL-33 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-33 Antibody [Alexa Fluor® 405]

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33 (interleukin 33; also NF-HEV and IL-1F11) is a 31-32 kDa member of the IL-1 family of proteins. It is expressed in endothelial cells and reportedly has two functions; first, it induces Th2-type cytokines, and second, it acts as a nuclear transcription factor. Human IL-33 is 270 amino acids (aa) in length. It contains a putative proregion (aa 1-111) and a mature segment (aa 112-270). The proregion contains a bipartite NLS (aa 61-78), plus an  alpha -helical homeodomain-like HTH motif (aa 1-65). The HTH motif mediates nuclear localization and heterochromatin association. A potential caspase 1 cleavage site exists in human IL-33 (Ser111-Ser112), but not in IL-33 from canine, porcine or bovine. There is one splice variant that shows a deletion of aa 113-118. Over aa 1-111, human IL-33 propeptide is 45% aa identical to mouse IL-33 propeptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.