IL-33 Antibody (6H617) - BSA Free

Western Blot: IL-33 Antibody (6H617) [NBP2-27333] - Analysis of IL-33 using IL-33 monoclonal antibodyin A) HUVEC cell lysate at 10 ug/mL and B) partial recombinant IL-33 (amino acids 112-270) at 1 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: IL-33 Antibody (6H617) [NBP2-27333] - An intracellular stain was performed on Jurkat cells with IL-33 (6H617) antibody NBP2-27333PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: IL-33 Antibody (6H617) [NBP2-27333] - Analysis of IL-33. Jurkat cells (0.5 x 10^6) were intracellularly stained with 0.25 ug of mouse IgG1 isotype control (green) or purified IL-33 antibody (Clone 6H617; red). This antibody and an anti-mouse IgG PE conjugated secondary antibody were used.

Flow Cytometry: IL-33 Antibody (6H617) [NBP2-27333] - An intracellular stain was performed on Jurkat cells with IL-33 [6H617] Antibody NBP2-27333F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 6H617
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IL-33 Antibody (6H617) - BSA Free Summary

• Immunogen: Full length recombinant protein
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL33
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/0.5x10^6 cells
  • Western Blot 1 - 10 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IL-33 Antibody (6H617) - BSA Free

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33 is a 270 amino acid highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. It is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAk1, IRAk4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. It is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-33 Antibody (NBP2-27333)(2)

Publications using NBP2-27333

• Di Sano C, D'Anna C, Ferraro M et Al. Impaired activation of Notch-1 signaling hinders repair processes of bronchial epithelial cells exposed to cigarette smoke Toxicol. Lett. 2020-03-10 [PMID: 32169443] (FLOW, Human)
• Kinchen J, Chen HH, Parikh K et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell 2018-09-25 [PMID: 30270042]

Bioinformatics

• Gene Symbol: IL33
• Uniprot:
  • O95760()