IL-33 Antibody (12B3C4)

Western Blot: IL-33 Antibody (12B3C4) [NBP1-75516] - Analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 ug/mL.

Immunocytochemistry/Immunofluorescence: IL-33 Antibody (12B3C4) [NBP1-75516] - Human lymph node tissue with IL-33 antibody at 20 ug/mL.

Immunohistochemistry-Paraffin: IL-33 Antibody (12B3C4) [NBP1-75516] - Human lymph node tissue with IL-33 antibody at 5 ug/mL.

Product Discontinued

IL-33 Antibody (12B3C4) Summary

• Immunogen: Antibody was raised against the recombinant IL-33 protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL33
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 22777948). Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IL-33 Antibody (12B3C4)

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1^5;^6;, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-_4;B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-33 Antibody (NBP1-75516)(4)

Publications using NBP1-75516

• Toskas A, Milias S, Delis G et al. Expression of IL-21 and IL-33 in Intestinal Mucosa of Inflammatory Bowel Disease: An Immunohistochemical Study Diagnostics 2023-06-27 [PMID: 37443579] (Immunohistochemistry-Paraffin, Human)
• Caglayan Sozmen S, Karaman M, Cilaker Micili S et al. Effects of Quercetin Treatment on Epithelium-derived Cytokines and Epithelial Cell Apoptosis in Allergic Airway Inflammation Mice Model. Iran J Allergy Asthma Immunol [PMID: 28129681] (IF/IHC)
• Sozmen SC, Karaman M, Micili SC et al. Resveratrol ameliorates 2, 4-dinitrofluorobenzene-induced atopic dermatitis-like lesions through effects on the epithelium. PeerJ. 2016-03-31 [PMID: 27069818] (IF/IHC, Mouse)
• Katwa, P, Wang, X, Urankar, R N et al. A Carbon Nanotube Toxicity Paradigm Driven by Mast Cells and the IL-33/ST2 Axis Small [PMID: 22777948] (IF/IHC, ICC/IF, Mouse)

FAQs for IL-33 Antibody (NBP1-75516). (Showing 1 - 2 of 2 FAQs).

1. Would you recommend a specific control for IHC-P?
   • Our antigen retrieval and IHC-P protocols can be found at the following links: Antigen Retrieval Protocol and Immunohistochemistry-Paraffin Protocol. I would recommend consulting our publications for this antibody for a positive control or testing at the Human Protein Atlas using a different antibody to this target for a positive control tissue.
2. I just bought the IL33 antibody, NBP1-7516. The dilution stated on the datasheet is 10;2000 for IHC-P. This seems a wide range. I need to optimize it firs before use. Can you advice on a dilution titre please.
   • For IHC-P, we recommend a concentration of 5ug/mL for NBP1-75516.

Bioinformatics

• Gene Symbol: IL33
• Uniprot:
  • NP_254274(Human)