Recombinant Human IL-32 Protein

Product Discontinued

Recombinant Human IL-32 Protein Summary

• Description: Recombinant bioactive protein for Human IL-32
  
  

  Source:E. coli
  Amino Acid Sequence:(P24001)

• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: Human IL-32 a activity is measured via the dose-dependent induction of TNF-a in the human THP-1 monocytic cell line.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL32
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 14.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: Lyophilized from 50 mM Sodium phosphate, (pH 7.5).
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with sterile H2O at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Alternate Names for Recombinant Human IL-32 Protein

• IL32
• IL-32
• IL-32alpha
• IL-32beta
• interleukin 32
• interleukin-32 theta
• interleukin-32
• natural killer cell transcript 4
• Natural killer cells protein 4
• NK4
• NK4IL-32delta
• TAIF
• TAIFa
• TAIFb
• TAIFc
• TAIFd
• TAIFIL-32gamma
• Tumor necrosis factor alpha-inducing factor

Background

Human IL-32alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-RBeta . IL-32alpha has been shown to induce IL-8, TNF-alpha, and MIP-2 production from human & mouse macrophage cell lines. It is up-regulated in activated T- & NK-cells, and IFN-g-treated epithelial cells. Human recombinant IL-32alpha produced in E. coli is a 131 amino acid non-glycosylated polypeptide with a molecular mass of 14.9 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL32