IL-32 Antibody

Product Discontinued

IL-32 Antibody Summary

• Immunogen: The amino acid sequence PELTPLLEKERDGLRC was used as the immunogen
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL32
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2 ug/ml

Reactivity Notes

The amino acid sequence used is 83% homologous in mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IL-32 Antibody

• IL32
• IL-32
• IL-32alpha
• IL-32beta
• interleukin 32
• interleukin-32 theta
• interleukin-32
• natural killer cell transcript 4
• Natural killer cells protein 4
• NK4
• NK4IL-32delta
• TAIF
• TAIFa
• TAIFb
• TAIFc
• TAIFd
• TAIFIL-32gamma
• Tumor necrosis factor alpha-inducing factor

Background

IL32 alfa is the shortest of the 4 isotypes of IL32 known, it is 134 amino acids ( kDa) protein, the IL32B (beta) is 188, IL32C is 168 and IL32D is 179 amino acids long proteins. Each subtype has an N-terminal segment and four kringle domains (NK4), that interact with several other proteins including the hepatic growth factor via c-met a tyrosine receptor kinase (5). IL-32 synergized with the intracellular nuclear oligomerization domain receptors (NOD1- and NOD2-specific muropeptides of peptidoglycans for the release of IL-1beta and IL-6. In contrast, IL-32 did not influence the cytokine production induced via TLRs (6). The anti-IL32 selective antibodies were made against an epitope that lies near the C-terminal end of the protein. The antibodies to IL32 are affinity purified on immobilized affinity based chromatography and characterized for applications in ELISA and Western blotting. The antiIL32 antibodies recognize a single band of IL32 in PC-IL32 samples. The IL32 antibodies do not cross react with other pro-inflammatory or anti-inflammatory interleukins, has also produced antibodies to other interleukins, interleukin receptors, cytokines and their receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-32 Antibody (NBP2-24774)(1)

Publication using NBP2-24774

• Ouhara K, Kawai T, Silva MJ et al. Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis. J Oral Microbiol. 2012-01-01 [PMID: 22435084] (Human)
  
  Details:
  Products cited: IL-32 (IMX-5871) for ELISA (used as a detection antibody in a sandwich ELISA): Fig 1A: healthy and inflamed human gingival tissue. IL-32 was down regulated in inflamed gingival tissue. Fig 2b: cultured medium supernatant from human gingiva

Bioinformatics

• Gene Symbol: IL32
• Uniprot:
  • P24001()