IL-31 Antibody (8-C3)

Western Blot: IL-31 Antibody (8-C3) [NBP2-80396] - Analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: IL-31 Antibody (8-C3) [NBP2-80396] - Staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IL-31 Antibody (8-C3) [NBP2-80396] - Analysis in human spleen tissue using IL-31 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: IL-31 Antibody (8-C3) [NBP2-80396] - Staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: IL-31 Antibody (8-C3) [NBP2-80396] - Staining IL-31 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IL-31 Antibody (8-C3) [NBP2-80396] - Analysis in human tonsil tissue using IL-31 antibody. Counter stained with hematoxylin.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: 8-C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

IL-31 Antibody (8-C3) Summary

• Immunogen: Recombinant protein within Human IL-31 aa 1-164 / 164. (SwissProt: Q6EBC2 Human)
• Localization: Secreted.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL31
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IL-31 Antibody (8-C3)

• IL31
• IL-31
• IL-31interleukin-31
• interleukin 31

Background

IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting ofIL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma(Dillon et al., 2004 (PubMed 15184896)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-31 Antibody (NBP2-80396)(1)

Publication using NBP2-80396

• Kim SM, Lee H, Lee ES Correlation of HLA-Cw6 Positivity with Clinical Characteristics and Treatment Efficacy in Korean Patients with Psoriasis Annals of dermatology 2022-08-01 [PMID: 35948327] (IHC-P, Human)
  
  Details:
  Dilution used for IHC 1:100

Bioinformatics

• Gene Symbol: IL31