IL-31 Antibody (308202) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 308202
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

IL-31 Antibody (308202) [Alexa Fluor® 594] Summary

• Specificity: Detects human IL‑31 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL‑31 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-31 Antibody (308202) [Alexa Fluor® 594]

• IL31
• IL-31
• IL-31interleukin-31
• interleukin 31

Background

Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein (1, 2). The mature region shows four alpha ‑helices which would be expected to show a typical up‑up‑down‑down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region (1). IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL‑31 RA (also GPL and GLM‑R) and the 180 kDa oncostatin M receptor (OSM R beta ) (2‑6). In the complex, IL-31 directly binds to GPL, not OSM R (2, 3). IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway (2‑5). Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable (2, 3). The IL-31 receptor is constitutively expressed by keratinocytes and up‑regulated by IFN-gamma on monocytes (1). Studies using transgenic mice indicate that IL-31 may contribute to the pruritis (itching) associated with nonatopic dermatitis (1, 7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.