IL-24 Antibody - BSA Free

Western Blot: IL-24 Antibody [NB100-56407] - Analysis of IL-24 in the A) absence and B) presence of blocking peptide in Jurkat cell lysate using this antibody at 0.5 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: IL-24 Antibody [NB100-56407] - Cell surface flow cytometry analysis of IL-24 in Jurkat cells using this antibody at 5 ug/10^6 cells. The shaded histogram represents cells only, green represents isotype control antibody, and red represents this antibody.

• Reactivity: Hu, Pm
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IL-24 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IL-24 Antibody - BSA Free (NB100-56407) is a polyclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Amino acids 123-136 (KNYHNRTVEVRTLK) of human IL-24 were used as the immunogen for this antibody.
• Specificity: The amino acid sequence used as immunogen is found in many of the IL-24 isoforms, including isoforms of 219, 207, 206, 154, 143 and 120 amino acids.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL24
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2-5 ug/10^6 cells
  • Western Blot 0.5-3 ug/ml
• Control:

  Jurkat Whole Cell Lysate
  IL-24 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IL-24 Antibody - BSA Free

• C49A
• FISP
• IL10B
• IL24
• IL-24
• interleukin 24
• MDA-7
• MDA7MOB5
• melanocyte-associated Mda-7
• melanoma differentiation association protein 7
• Melanoma differentiation-associated gene 7 protein
• MOB-5
• ST16
• ST16interleukin-24
• suppression of tumorigenicity 16 (melanoma differentiation)
• Suppression of tumorigenicity 16 protein

Background

Interleukin 24 (IL-24)/melanoma differentiation-associated gene-7 (mda-7) encodes a secreted protein that exhibits significant homology to the interleukin 10 (IL-10) family of cytokines. The human IL-24 is secreted by activated peripheral blood mononuclear cells and binds two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2 resulting in the activation of JAK/STAT signaling pathways. Mda-7/IL-24, selectively induces growth suppression, apoptosis and radio sensitization in malignant gliomas in a p53-independent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL24
• Entrez:
  • 11009(Human)