IL-23R Antibody (753317) [Alexa Fluor® 700]

Mouse splenocytes treated with 10 µg/mL Anti-CD3 (MAB484), 5 µg/mL Anti-CD28 (AF483), 10 ng/mL Recombinant Mouse TGF-beta 1 (Catalog # 100-B), 20 ng/mL Recombinant Mouse IL-23 (1887-ML), 40 ng/mL Recombinant Mouse IL-6 (406-ML), and 10 ng/mL Recombinant Mouse IL-1 beta (401-ML) for 5 days to induce T17 activation were stained with Rat Anti-Mouse CD4 Fluorescein-conjugated Monoclonal Antibody (FAB554F) and either (A) Rat Anti-Mouse IL-23 R Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB16861N) or (B) Rat IgG1Alexa Fluor 700 Isotype Control (Catalog # IC005N). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL-23 R Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB16861N, filled histogram) or isotype control antibody (IC005N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

IL-23R Antibody (753317) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R
  Gly24-Asp372
  Accession # Q8K4B4
• Specificity: Detects endogenous mouse IL-23 R by flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL23R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23R Antibody (753317) [Alexa Fluor® 700]

• IBD17
• IL-23 R
• IL-23 receptor
• IL23R
• IL-23R
• interleukin 23 receptor
• interleukin-23 receptor

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4⁺ CD45RB^low memory T cells but at much lower levels by mouse CD4⁺ CD45RB^high cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5448.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23R Antibody (FAB16861N)(2)

Publications using FAB16861N

• I Dey, B Bishayi Impact of simultaneous neutralization of IL-17A and treatment with recombinant IL-2 on Th17-Treg cell population in S.aureus induced septic arthritis Microb. Pathog., 2019-11-29;139(0):103903. 2019-11-29 [PMID: 31790794] (ICC, Mouse)
• Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis. Nature, 2015-03-30;522(7556):345-8. 2015-03-30 [PMID: 25822788] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL23R
• Uniprot:
  • Q8K4B4()