IL-23R Antibody (218213) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) (A) cultured with plate-bound 5 μg/mL anti-Human CD3, 2 μg/mL anti-Human CD28 (AF-342-PB), 20 ng/mL Recombinant Human IL-2 (202-GMP), 10 ng/mL TGF-beta (100-B), 20 ng/mL Recombinant Human IL-23 (1290-IL), 40 ng/mL Recombinant Human IL-6 (206-IL), 10 ng/mL Recombinant Human IL-1 beta (201-LB), and 10 μg/mL anti-Human IFN-gamma (AF-285-NA) for 7 days to induce Th17 development or (B) resting, were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A) and Mouse Anti-Human IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB14001P). Quadrant markers were set based on Mouse IgG2B Isotype Control (IC0041P). Staining was performed using our Staining Membrane-associated Proteins protocol.

HEK293 human cell line transfected with human IL-23 R were stained with Mouse Anti-Human IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB14001P, filled histogram) or Mouse IgG2B Isotype Control (IC0041P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Product Discontinued

IL-23R Antibody (218213) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-23 R
  Gly24-Ile354
  Accession # Q5VWK5
• Specificity: Detects human IL-23 R in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑23 R.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL23R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23R Antibody (218213) [Phycoerythrin]

• IBD17
• IL-23 R
• IL-23 receptor
• IL23R
• IL-23R
• interleukin 23 receptor
• interleukin-23 receptor

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5448.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23R Antibody (FAB14001P)(16)

Publications using FAB14001P

• Najar, M;Rahmani, S;Faour, WH;Alsabri, SG;Lombard, CA;Fayyad-Kazan, H;Sokal, EM;Merimi, M;Fahmi, H; Umbilical Cord Mesenchymal Stromal/Stem Cells and Their Interplay with Th-17 Cell Response Pathway Cells 2024-01-16 [PMID: 38247860] (Flow Cytometry, Human)
• M Najar, M Merimi, WH Faour, CA Lombard, D Moussa Agh, Y Ouhaddi, EM Sokal, L Lagneaux, H Fahmi In Vitro Cellular and Molecular Interplay between Human Foreskin-Derived Mesenchymal Stromal/Stem Cells and the Th17 Cell Pathway Pharmaceutics, 2021-10-19;13(10):. 2021-10-19 [PMID: 34684029] (Flow Cytometry, Human)
• Samarani S, Sagala P, Jantchou P, Grimard G, Faure C, Deslandres C, Amre D, Ahmad A Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients. Mediators Inflamm, 2020-02-19;2020(0):6401969. 2020-02-19 [PMID: 32148442] (Flow Cytometry, Human)
• Rui Sun, Matija Hedl, Clara Abraham IL23 induces IL23R recycling and amplifies innate receptor-induced signalling and cytokines in human macrophages, and the IBD-protective IL23R R381Q variant modulates these outcomes Gut 2/1/2020 [PMID: 31097538]
• Amardeep Singh, Yousif Subhi, Marie Krogh Nielsen, Mads Krüger Falk, Sara Maj Hyldig Matzen, Finn Sellebjerg et al. Systemic frequencies of T helper 1 and T helper 17 cells in patients with age-related macular degeneration: A case-control study Scientific Reports 4/4/2017 [PMID: 28377586]
• Suxia Bao Role of interleukin-23 in monocyte-derived dendritic cells of HBV-related acute-on-chronic liver failure and its correlation with the severity of liver damage Clin Res Hepatol Gastroenterol, 2016-12-29;0(0):. 2016-12-29 [PMID: 28041935] (Flow Cytometry, Human)
• Lars Fugger Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity Sci Transl Med, 2016-11-02;8(363):363ra149. 2016-11-02 [PMID: 27807284] (Flow Cytometry, Human)
• Dawn C. Newcomb, Jacqueline Yvonne Cephus, Madison G. Boswell, John M. Fahrenholz, Emily W. Langley, Amy S. Feldman et al. Estrogen and progesterone decrease let-7f microRNA expression and increase IL-23/IL-23 receptor signaling and IL-17A production in patients with severe asthma Journal of Allergy and Clinical Immunology 10/1/2015 [PMID: 26242299]
• Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha. Eur J Immunol, 2015-01-19;45(4):1092-102. 2015-01-19 [PMID: 25545357] (FACS, Human)
• Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination. Eur J Immunol, 2015-02-01;45(2):442-51. 2015-02-01 [PMID: 25141829] (Flow Cytometry, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 2012-09-25;10(9):e1001395. 2012-09-25 [PMID: 23055831] (Flow Cytometry, Human)
• Cocco C, Di Carlo E, Zupo S Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo. Leukemia, 2011-12-23;26(6):1365-74. 2011-12-23 [PMID: 22193967] (Flow Cytometry, Human)
• Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA Identification of human NK17/NK1 cells. PLoS ONE, 2011-10-21;6(10):e26780. 2011-10-21 [PMID: 22039549] (Flow Cytometry, Human)
• Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes. J. Immunol., 2011-03-02;186(7):4175-82. 2011-03-02 [PMID: 21368235] (Flow Cytometry, Human)
• Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol. Lett., 2010-10-15;134(2):113-21. 2010-10-15 [PMID: 20951740] (Flow Cytometry, Human)
• de Paus RA, van de Wetering D, Van Dissel JT IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants. Mol. Immunol., 2008-09-01;45(15):3889-95. 2008-09-01 [PMID: 18675459] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL23R
• Uniprot:
  • Q5VWK5()