Recombinant Mouse IL-2 Animal-Free Protein

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SDS-Page: Recombinant Mouse IL-2 Animal-Free Protein [NBP2-76177] - 1 ug run under (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Mouse IL-2 Animal-Free Protein [NBP2-76177] - IL-2 induced proliferation of STLL-2 cells.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Animal Free
Concentration
LYOPH

Order Details

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Recombinant Mouse IL-2 Animal-Free Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Mouse IL-2

Source: E. coli

Amino Acid Sequence: MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSIISTSPQ

Specificity
IL-2 is a monomer, 17.4 kDa (150 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
CTLL-2 cell proliferation, >2 x 10^5 units/mg (ED50 acceptance criteria are <5 ng/mL).
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL2
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium citrate, pH 4.0
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile 10 mM acetic acid at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Mouse IL-2 Animal-Free Protein

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • Proleukin
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL2 may be of use as an anti-tumor agent in cancer therapy. Recombinant human IL2 is a 15.5 kDa protein, consisting of 134 amino acid residues. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL2