Recombinant Canine IL-2 His Protein

Bioactivity: Recombinant Canine IL-2 Protein [NBP2-59565] - Canine IL-2 stimulates cell cytotoxicity in the CTLL-2 mouse cytotoxic T cell. The ED50 range = 0.3ng/ml.

SDS-Page: Recombinant Canine IL-2 Protein [NBP2-59565] - 15% SDS-PAGE (3ug)

• Reactivity: Ca
• Applications: PAGE, Bioactivity
• Concentration: 0.5 mg/ml

Recombinant Canine IL-2 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-155 of Canine IL-2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAPITS SSTKETEQQM EQLLLDLQLL LNGVNNYENP QLSRMLTFKF YTPKKATEFT HLQCLAEELK NLEEVLGLPQ SKNVHLTDTK ELISNMNVTL LKLKGSETSY NCEYDDETAT ITEFLNKWIT FSQSIFSTLT

• Details of Functionality: Canine IL-2 stimulates cell cytotoxicity in the CTLL-2 mouse cytotoxic T cell. The ED50 range is less than or equal to 0.3ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL2
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 18.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Canine IL-2 His Protein

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL2 may be of use as an anti-tumor agent in cancer therapy. Recombinant human IL2 is a 15.5 kDa protein, consisting of 134 amino acid residues. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL2