IL-2 R beta Antibody (130122)

Mouse splenocytes were stained with anti-mouse Integrin alpha 2/CD49b APC‑conjugated antibody and either (A) Rat Anti-Mouse IL‑2 R beta Monoclonal Antibody (Catalog # MAB5891) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 130122
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-2 R beta Antibody (130122) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-2 R beta
  Ala27-Glu240
  Accession # P16297
• Specificity: Detects mouse IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha , rhIL-2 R beta , recombinant mouse (rm) IL-2 R gamma , rhIL-15 R alpha , or rmIL-21 R is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL2RB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 R beta Antibody (130122)

• CD122 antigen
• CD122
• high affinity IL-2 receptor beta subunit
• High affinity IL-2 receptor subunit beta
• IL-15 R beta
• IL-2 R beta
• IL-2 receptor subunit beta
• IL2R beta
• IL-2R subunit beta
• IL2RB
• IL-2Rb
• interleukin 15 receptor, beta
• interleukin 2 receptor, beta
• interleukin-2 receptor subunit beta
• p70-75
• p75

Background

IL‑2 R beta (IL-2 Receptor beta ; designated CD122) is a type I transmembrane protein that associates with IL-2 R alpha and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. IL‑2 R beta and the common gamma chain can also associate with IL-15 R alpha . IL-2 R beta is expressed by T, B, NK cells and monocyte/macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RB
• Uniprot:
  • P16297()