IL-2 ELISA Development Kit Summary
| Description |
The ferret IL-2 ELISA development kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of ferret IL-2 in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP, ELISA standard and standard reconstitution buffer. Components are sufficient for 6 ELISA plates (96-wells each). Plates and substrate are not included. |
| Standard Curve Range |
3 - 1000 pg/mL |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Development Kit |
| Gene |
IL2 |
Applications/Dilutions
Reactivity Notes
The monoclonal antibodies in this kit react with porcine IL-2 and cross-react with IL-2 from ferret. The ELISA standard supplied is recombinant porcine IL-2.
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated detection mAb (MT265)
- Capture mAb (MT264)
- Recombinant porcine IL-2 ELISA standard
- Standard reconstitution buffer
- Streptavidin-HRP
|
Alternate Names for IL-2 ELISA Development Kit
Background
Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL2 may be of use as an anti-tumor agent in cancer therapy. Recombinant human IL2 is a 15.5 kDa protein, consisting of 134 amino acid residues. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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