Mouse IL-2 ELISA Kit (Colorimetric) Summary
Description |
The mouse IL-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-2. |
Specificity |
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-2 positive serum. There was no crossreactivity detected. |
Standard Curve Range |
15.6 - 1,000 pg/mL |
Sensitivity |
5.3 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 0.1 |
Intra-Assay |
CV% < 5.00% |
Spike Recovery |
119% |
Sample Volume |
50 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
IL2 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse sCD40L
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-mouse sCD40L monoclonal antibody
- Mouse sCD40L Standard lyophilized, 40 ng/ml upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
|
Alternate Names for Mouse IL-2 ELISA Kit (Colorimetric)
Background
Interleukin-2 (IL-2) plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. Antigen-specific proliferation of helper and cytotoxic T-lymphocytes following stimulation is critically dependent on IL-2 expression, secretion, and binding to receptors in an autocrine fashion on the surface of T cells. Apart from its most important role to mediate antigen specific T lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B and NK cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. Alterations in the ability of T cells to synthesize IL-2 have been observed in physiologic and pathologic states.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IL-2 ELISA Kit (NBP1-92661). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.