Human IL-2 ELISA Kit (Colorimetric)

ELISA: IL-2 ELISA Kit (Colorimetric) [NBP1-89875] - Representative standard curve for human IL-2 ELISA. Human IL-2 was diluted in serial 2-fold steps in SampleDiluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Standard Curve Range: 18.8 - 1,200 pg/mL
• Sensitivity: 9.9 pg/mL

Human IL-2 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-2 positive serum. There was no cross reactivity or interference detected.
• Standard Curve Range: 18.8 - 1,200 pg/mL
• Sensitivity: 9.9 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.0%
• Intra-Assay: CV% < 7.0%
• Spike Recovery: See protocol for Spike recovery results.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-2 ELISA Kit (Colorimetric)

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

IL-2 plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. IL-2 is a 15 kDa glycoprotein encoded by a single gene located in the q26-28 region of human chromosome 4. The cDNA deduced polypeptide consists of 153 amino acids. Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. IL-2 displays antitumoral effects, thus being used in cancer therapy. Monitoring of IL-2 levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL2