Human IL-2 ELISA Kit (Colorimetric)

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ELISA: IL-2 ELISA Kit (Colorimetric) [NBP1-89875] - Representative standard curve for human IL-2 ELISA. Human IL-2 was diluted in serial 2-fold steps in SampleDiluent. Do not use this standard curve to derive test ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine, white blood
Standard Curve Range
18.8 - 1,200 pg/mL
Sensitivity
9.9 pg/mL

Order Details

Human IL-2 ELISA Kit (Colorimetric) Summary

Specificity
The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-2 positive serum. There was no cross reactivity or interference detected.
Standard Curve Range
18.8 - 1,200 pg/mL
Sensitivity
9.9 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.0%
Intra-Assay
7.0%
Spike Recovery
See protocol for Spike recovery results.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL2

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human BTLA

Alternate Names for Human IL-2 ELISA Kit (Colorimetric)

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

IL-2 plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. IL-2 is a 15 kDa glycoprotein encoded by a single gene located in the q26-28 region of human chromosome 4. The cDNA deduced polypeptide consists of 153 amino acids. Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. IL-2 displays antitumoral effects, thus being used in cancer therapy. Monitoring of IL-2 levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL2
Entrez