IL-2 Antibody (100312) [Allophycocyanin]

Porcine peripheral blood mononuclear cells stimulated with LPS were stained with Mouse Anti-Porcine IL-2 APC-conjugated Monoclonal Antibody (Catalog # IC6521A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.

Product Discontinued

IL-2 Antibody (100312) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant porcine IL-2
  Ala21-Thr154
  Accession # P26891
• Specificity: Detects porcine IL-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with human, mouse, rat, bovine, canine, equine, feline, or cotton rat IL-2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody (100312) [Allophycocyanin]

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma _c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4⁺ and CD8⁺ T cells, gamma δ T cells, eosinophils, endothelial cells, and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8⁺ T cell proliferation, and promote NK cell and CD8⁺ T cell effector activity. IL-2 also appears to drive CD4⁺ Fox P3^- thymocytes into mature FoxP3⁺ Tregs, and to direct the conversion of CD4⁺ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma , and endothelial cells to upregulate endocytic activity. Mature porcine IL-2 shares 71% and 51% amino acid sequence identity with human and mouse IL-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • P26891()