IL-18R alpha/IL-1 R5 Antibody (112614) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB1216, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clone: 112614
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-18R alpha/IL-1 R5 Antibody (112614) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL‑1 R5
  Ser19-Gly326
  Accession # Q61098
• Specificity: Detects mouse IL‑18 R alpha /IL‑1 R5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, recombinant human (rh) IL-1 R3, rhIL-1 R4, recombinant rat (rr) IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Il18rap
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18R alpha/IL-1 R5 Antibody (112614) [Unconjugated]

• CD218a
• CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1
• IL-1 R5
• IL-1 Rrp
• IL-18 R alpha
• IL-18 R1
• IL18R alpha
• IL18R1
• IL-18Ra

Background

IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18R alpha/IL-1 R5 Antibody (MAB1216)(1)

Publication using MAB1216

• Alboni S, Cervia D, Ross B, Montanari C, Gonzalez AS, Sanchez-Alavez M, Marcondes MC, De Vries D, Sugama S, Brunello N, Blom J, Tascedda F, Conti B Mapping of the full length and the truncated interleukin-18 receptor alpha in the mouse brain. J. Neuroimmunol., 2009-07-28;214(1):43-54. 2009-07-28 [PMID: 19640592] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: Il18rap
• Uniprot:
  • Q61098()