IL-18 BPa/IL18BP Antibody [Unconjugated]

Western blot shows conditioned media from HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 50 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) and 40 ng/mL Recombinant Human TNF-alpha (210-TA) for 48 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-18 BPa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Human IL-18 BPa Fc Chimera (119-BP) inhibits Recombinant Human IL-18/IL-1F4 induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). Inhibition of Recombinant Human IL-18/IL-1F4 (40 ng/mL) activity elicited by Recombinant Human IL-18 BPa Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119). The ND50 is typically 1-5 µg/mL in the presence of Recombinant Human TNF-alpha (20 ng/mL).

Recombinant Human IL-18/IL-18 BPa complex protein was serially diluted 2-fold and captured by Rabbit Anti-Human IL-18/IL-1F4 Monoclonal Antibody (Catalog # MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: WB, ELISA, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-18 BPa/IL18BP Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-18 BPa
  Thr31-Gly194
  Accession # O95998
• Specificity: Detects human IL‑18 BPa in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL18BP
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Neutralization 1-5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18 BPa/IL18BP Antibody [Unconjugated]

• IL18 BPa
• IL-18 BPa
• IL18BP
• IL-18BP
• IL18BPa
• interleukin 18 binding protein
• interleukin-18-binding protein
• MC51L-53L-54L homolog gene product
• tadekinig-alfa

Background

Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma , suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
3. Calderara, S. et al. (2001) Virology 279:22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18 BPa/IL18BP Antibody (AF119)(4)

Publications using AF119

• Jeerawattanawart, S;Hansakon, A;Roytrakul, S;Angkasekwinai, P; Regulation and function of adiponectin in the intestinal epithelial cells in response to Trichinella spiralis infection Scientific reports 2023-08-27 [PMID: 37635188] (Neutralization, Mouse)
• Serkan Belkaya, Eleftherios Michailidis, Cecilia B. Korol, Mohammad Kabbani, Aurélie Cobat, Paul Bastard et al. Inherited IL-18BP deficiency in human fulminant viral hepatitis Journal of Experimental Medicine 8/5/2019 [PMID: 31213488]
• Sallmon H, Hoene V, Weber SC, Dame C Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system. J. Interferon Cytokine Res., 2010-02-01;30(2):55-8. 2010-02-01 [PMID: 20028206] (Western Blot, Human)
• Fantuzzi G, Banda NK, Guthridge C, Vondracek A, Kim SH, Siegmund B, Azam T, Sennello JA, Dinarello CA, Arend WP Generation and characterization of mice transgenic for human IL-18-binding protein isoform a. J. Leukoc. Biol., 2003-08-11;74(5):889-96. 2003-08-11 [PMID: 12960225] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL18BP
• Uniprot:
  • O95998()