IL-18 BPa/IL18BP Antibodies

285-IF) and 40 ng/mL Recombinant Human TNF-alpha (210-TA) for 48 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-18 BPa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 285-IF) and 40 ng/mL Recombinant Human TNF-alpha (210-TA) for 48 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-18 BPa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media from HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 50 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) and 40 ng/mL Recombinant Human TNF-alpha (210-TA) for 48 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-18 BPa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

119-BP) inhibits Recombinant Human IL-18/IL-1F4 induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). Inhibition of Recombinant Human IL-18/IL-1F4 (40 ng/mL) activity elicited by Recombinant Human IL-18 BPa Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119). The ND₅₀ is typically 1-5 µg/mL in the presence of Recombinant Human TNF-alpha (20 ng/mL)." class="big_lightbox"> 119-BP) inhibits Recombinant Human IL-18/IL-1F4 induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). Inhibition of Recombinant Human IL-18/IL-1F4 (40 ng/mL) activity elicited by Recombinant Human IL-18 BPa Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119). The ND₅₀ is typically 1-5 µg/mL in the presence of Recombinant Human TNF-alpha (20 ng/mL)." class="big_thumb" />

MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

119-BP) was serially diluted and captured by Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1192) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 119-BP) was serially diluted and captured by Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1192) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

119-BP) was serially diluted and captured by Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1192) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 119-BP) was serially diluted and captured by Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1192) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human IL‑18 BPa Fc Chimera (Catalog # 119-BP) was serially diluted and captured by Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1192) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

119-BP) inhibition of Recombinant Human IL-18/IL-1F4 (9124-IL) induced IFN gamma secretion in the KG-1 human acute myelogenous leukemia cell line as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). The ND₅₀ for this effect is typically 0.800-12.0 µg/mL." class="big_lightbox"> 119-BP) inhibition of Recombinant Human IL-18/IL-1F4 (9124-IL) induced IFN gamma secretion in the KG-1 human acute myelogenous leukemia cell line as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). The ND₅₀ for this effect is typically 0.800-12.0 µg/mL." alt="Mouse Anti-Human IL-18 BPa Monoclonal Antibody (Catalog # MAB1191) neutralizes Recombinant Human IL-18 BPa Fc Chimera (119-BP) inhibition of Recombinant Human IL-18/IL-1F4 (9124-IL) induced IFN gamma secretion in the KG-1 human acute myelogenous leukemia cell line as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). The ND₅₀ for this effect is typically 0.800-12.0 µg/mL." class="big_thumb" />