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285-IF) and 40 ng/mL Recombinant Human TNF-alpha (210-TA) for 48 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-18 BPa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
119-BP) inhibits Recombinant Human IL-18/IL-1F4 induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). Inhibition of Recombinant Human IL-18/IL-1F4 (40 ng/mL) activity elicited by Recombinant Human IL-18 BPa Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119). The ND50 is typically 1-5 µg/mL in the presence of Recombinant Human TNF-alpha (20 ng/mL)." class="big_lightbox" target="_blank">
MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox" target="_blank">
119-BP) was serially diluted and captured by Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1192) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
119-BP) was serially diluted and captured by Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1192) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
IL-18BP which binds to IL-18, also known as interferon-gamma inducing factor (IGIF) which is a pro-inflammatory cytokine that belongs to the IL-1 family (1). IL-18 is a pleiotropic factor involved in the regulation of both innate and acquired immune responses, playing a key role in autoimmune, inflammatory, and infectious diseases (2). Interleukin-18 binding protein (IL18-BP) is an inhibitor of the pro-inflammatory cytokine IL18. The recombinant forms of IL18BP did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity (3). IL-18 plays an important role in host defense against microbial pathogens. Many poxviruses encode homologous IL-18BP that neutralizes IL-18 activity. Data show that IL-18BP prevents IL-18 from binding to IL-18R by interacting with three residues that are part of the binding site for IL-18Ralpha (4).
![Western Blot IL-18 BPa/IL18BP Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL18_BPa_AF119_Western_Blot_14741.jpg)
![ELISA IL-18 BPa/IL18BP Antibody (136031) [Unconjugated]](http://images.novusbio.com/images/mab1192_human-il-18-bpa-mab-clone-136031-elisa-639201740030924478.jpg)
![Sandwich ELISA IL-18 BPa/IL18BP Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-18-BPa-IL18BP-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79673-img0001.jpg)
![N/A IL-18 BPa/IL18BP [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL18_BPa_DY119_ELISA_1598.jpg)
![SEC-MALS IL-18 BPa/IL18BP [Unconjugated]](http://images.novusbio.com/fullsize/11236-bp_recombinant-human-il-18-bpa-his-tag-protein-cf-sec-mals-2372025111833..jpg)
![Bioactivity IL-18 BPa/IL18BP [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL18_BPa_2523BP_2399.jpg)
![Neutralization IL-18 BPa/IL18BP Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-18_BPa_AF119_Block_Neutralize_8764.jpg)
![ELISA IL-18 BPa/IL18BP Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL18_BPa_AF119_ELISA_22266.jpg)
![ELISA IL-18 BPa/IL18BP Antibody [Unconjugated]](http://images.novusbio.com/images/af119_human-il-18-bpa-affinity-purified-polyclonal-ab-elisa-639201852920074508.jpg)
![ELISA IL-18 BPa/IL18BP Antibody [Unconjugated]](http://images.novusbio.com/images/af119_human-il-18-bpa-affinity-purified-polyclonal-ab-elisa-639201852920074518.jpg)
![Bioactivity IL-18 BPa/IL18BP [Unconjugated]](http://images.novusbio.com/fullsize/11236-bp_recombinant-human-il-18-bpa-his-tag-protein-cf-bioactivity-2482022152931.jpg)
![SDS-Page IL-18 BPa/IL18BP [Unconjugated]](http://images.novusbio.com/fullsize/11236-bp_recombinant-human-il-18-bpa-his-tag-protein-cf-sds-page-382022135813.jpg)
![SDS-Page IL-18 BPa/IL18BP [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL18_BPa_2523BP_2392.jpg)
