IL-18 BPa/IL18BP Antibody Pair [HRP] Summary
Description |
Solid Phase sandwich ELISA for the quantitative determination of Human IL-18 BPa/IL18BP. |
Specificity |
This solid phase sandwich ELISA utilizes an antibody specific for Human IL-18 BPa/IL18BP. |
Standard Curve Range |
46.88-3000 pg/ml (example only; lot dependent) |
Sensitivity |
46.88 pg/ml (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Sample Volume |
100 ul |
Gene |
IL18BP |
Applications/Dilutions
Dilutions |
- Immunoprecipitation 10 ug/mg lysate
- Sandwich ELISA
|
Application Notes |
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/ml is recommended. |
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Capture Antibody: 1 mg/ml of mouse anti-Human IL-18 BPa/IL18BP monoclonal antibody (in PBS, pH 7.4).
- Detection Antibody: 0.2 mg/ml of rabbit anti-Human IL-18 BPa/IL18BP polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
- Standard: Each vial contains 47 ng of recombinant Human IL-18 BPa/IL18BP.
|
Alternate Names for IL-18 BPa/IL18BP Antibody Pair [HRP]
Background
IL-18BP which binds to IL-18, also known as interferon-gamma inducing factor (IGIF) which is a pro-inflammatory cytokine that belongs to the IL-1 family (1). IL-18 is a pleiotropic factor involved in the regulation of both innate and acquired immune responses, playing a key role in autoimmune, inflammatory, and infectious diseases (2). Interleukin-18 binding protein (IL18-BP) is an inhibitor of the pro-inflammatory cytokine IL18. The recombinant forms of IL18BP did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity (3). IL-18 plays an important role in host defense against microbial pathogens. Many poxviruses encode homologous IL-18BP that neutralizes IL-18 activity. Data show that IL-18BP prevents IL-18 from binding to IL-18R by interacting with three residues that are part of the binding site for IL-18Ralpha (4).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are
guaranteed for 6 months from date of receipt.
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