IL-18 BPa/IL18BP Antibody Pair [HRP]

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Sandwich ELISA: IL-18 BPa/IL18BP Antibody Pair [HRP] [NBP2-79673] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP, S-ELISA, WB (-)
Conjugate
HRP
Standard Curve Range
46.88-3000 pg/ml (example only; lot dependent)
Sensitivity
46.88 pg/ml (example only; lot dependent)

Order Details

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Catalog# & Conjugate Size Price

IL-18 BPa/IL18BP Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human IL-18 BPa/IL18BP.
Specificity
This solid phase sandwich ELISA utilizes an antibody specific for Human IL-18 BPa/IL18BP.
Standard Curve Range
46.88-3000 pg/ml (example only; lot dependent)
Sensitivity
46.88 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Gene
IL18BP

Applications/Dilutions

Dilutions
  • Immunoprecipitation 10 ug/mg lysate
  • Sandwich ELISA
Application Notes
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 1 mg/ml of mouse anti-Human IL-18 BPa/IL18BP monoclonal antibody (in PBS, pH 7.4).
  2. Detection Antibody: 0.2 mg/ml of rabbit anti-Human IL-18 BPa/IL18BP polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  3. Standard: Each vial contains 47 ng of recombinant Human IL-18 BPa/IL18BP.

Alternate Names for IL-18 BPa/IL18BP Antibody Pair [HRP]

  • IL18 BPa
  • IL-18 BPa
  • IL18BP
  • IL-18BP
  • IL18BPa
  • interleukin 18 binding protein
  • interleukin-18-binding protein
  • MC51L-53L-54L homolog gene product
  • tadekinig-alfa

Background

IL-18BP which binds to IL-18, also known as interferon-gamma inducing factor (IGIF) which is a pro-inflammatory cytokine that belongs to the IL-1 family (1). IL-18 is a pleiotropic factor involved in the regulation of both innate and acquired immune responses, playing a key role in autoimmune, inflammatory, and infectious diseases (2). Interleukin-18 binding protein (IL18-BP) is an inhibitor of the pro-inflammatory cytokine IL18. The recombinant forms of IL18BP did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity (3). IL-18 plays an important role in host defense against microbial pathogens. Many poxviruses encode homologous IL-18BP that neutralizes IL-18 activity. Data show that IL-18BP prevents IL-18 from binding to IL-18R by interacting with three residues that are part of the binding site for IL-18Ralpha (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL18BP