IL-18 BPa/IL18BP Antibody Pair [HRP]

Sandwich ELISA: IL-18 BPa/IL18BP Antibody Pair [HRP] [NBP2-79673] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

• Reactivity: Hu
• Applications: IP, S-ELISA, WB (-)
• Conjugate: HRP
• Standard Curve Range: 46.88-3000 pg/ml (example only; lot dependent)
• Sensitivity: 46.88 pg/ml (example only; lot dependent)

IL-18 BPa/IL18BP Antibody Pair [HRP] Summary

• Description: Solid Phase sandwich ELISA for the quantitative determination of Human IL-18 BPa/IL18BP.
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human IL-18 BPa/IL18BP.
• Standard Curve Range: 46.88-3000 pg/ml (example only; lot dependent)
• Sensitivity: 46.88 pg/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: IL18BP

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10 ug/mg lysate
  • Sandwich ELISA
• Application Notes: 1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating.
  2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
  3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/ml is recommended.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for IL-18 BPa/IL18BP Antibody Pair [HRP]

• IL18 BPa
• IL-18 BPa
• IL18BP
• IL-18BP
• IL18BPa
• interleukin 18 binding protein
• interleukin-18-binding protein
• MC51L-53L-54L homolog gene product
• tadekinig-alfa

Background

IL-18BP which binds to IL-18, also known as interferon-gamma inducing factor (IGIF) which is a pro-inflammatory cytokine that belongs to the IL-1 family (1). IL-18 is a pleiotropic factor involved in the regulation of both innate and acquired immune responses, playing a key role in autoimmune, inflammatory, and infectious diseases (2). Interleukin-18 binding protein (IL18-BP) is an inhibitor of the pro-inflammatory cytokine IL18. The recombinant forms of IL18BP did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity (3). IL-18 plays an important role in host defense against microbial pathogens. Many poxviruses encode homologous IL-18BP that neutralizes IL-18 activity. Data show that IL-18BP prevents IL-18 from binding to IL-18R by interacting with three residues that are part of the binding site for IL-18Ralpha (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL18BP