IL-17RB Antibody [Unconjugated]

Western blot shows lysates of mouse liver tissue and mouse spleen tissue (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL-17 RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1040) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-17 RB at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL-17RB was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL-17RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1040) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17RB Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RB
  Arg18-Gly286
  Accession # Q9JIP3
• Specificity: Detects mouse IL‑17 RB in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-17 R and recombinant human IL-17 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL17RB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1.7-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RB Antibody [Unconjugated]

• CRL4IL-17 receptor homolog 1
• cytokine receptor CRL4
• Cytokine receptor-like 4
• Evi27
• EVI27IL-17B receptor
• IL-17 RB
• IL-17 receptor B
• IL-17 RH1
• IL17BRinterleukin 17B receptor
• IL-17ER
• IL17RB
• IL-17RB
• IL17Rh1
• IL-17Rh1
• IL17RH1MGC5245
• interleukin 17 receptor B
• interleukin 17 receptor homolog 1
• interleukin-17 receptor B
• Interleukin-17B receptor

Background

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2). Interleukin-17B Receptor (IL-17 RB), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL17B R cDNA encodes a 499 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17 RB also exists (4). Human and mouse IL‑17 RB share 76% aa sequence identity. The IL-17 RB protein sequence is only 19.2% and 18.2% identical to the human and mouse IL-17 R sequence, respectively. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL‑17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17 RB is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2‑4). The expression of IL-17 RB is significantly up‑regulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2‑4).

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
4. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
5. Tian, E. et al. (2000) Oncogene 19:2098.
6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
7. Hurst, S.D. et al. (2002) J. Immunol. 169:443.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RB Antibody (AF1040)(2)

Publications using AF1040

• Fang Zhang, Ye Liu, Shiqi Wang, Xin Yan, Yue Lin, Deyan Chen et al. Interleukin-25-Mediated-IL-17RB Upregulation Promotes Cutaneous Wound Healing in Diabetic Mice by Improving Endothelial Cell Functions Frontiers in Immunology 2022-01-20 [PMID: 35126394]
• M Zaremba-Cz, A Hryniewicz, R Tabola, M Nienartowi, K Stach, J Wierzbicki, R Cirocchi, P Ziolkowski, S Tabaczar, K Augoff A novel regulatory function of CDKN1A/p21 in TNF?-induced matrix metalloproteinase 9-dependent migration and invasion of triple-negative breast cancer cells Cell. Signal., 2018-03-26;47(0):27-36. 2018-03-26 [PMID: 29588220] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: IL17RB
• Uniprot:
  • Q9JIP3()