Recombinant Human IL-17F Protein

SDS-Page: Human IL-17F Protein [NBP2-35046]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-17F Protein Summary

• Description: A disulfide-linked homodimer of two 134 amino acid polypeptide chains corresponding to IL-17F Source: E. coli

  Uniprot ID: Q96PD4

  Amino Acid Sequence: MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ

• Details of Functionality: IL-17F protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 x 10^4 IU/mg
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL17F
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-17F as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 30.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in 6 mM HCl to a concentration of 0.1mg/ml.

Alternate Names for Recombinant Human IL-17F Protein

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• interleukin 17F

Background

Human Interleukin-17F (IL-17F) is encoded by the IL17F gene located on the chromosome 6 and belongs to the IL-17 family which contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17F that shares 50 % homologous of crystal structure to IL-17A and is expressed by activated T cells, has the functions of stimulating production of other cytokines (such as IL-6, IL-8 and granulocyte colony-stimulating factor) and proliferation of PBMC and T-cell, regulating cartilage matrix turnover, and inhibiting angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL17F
• Uniprot:
  • Q96PD4()
  • Q96PD4()
  • Q96PD4()