Recombinant Mouse IL-17F Protein

Product Discontinued

Recombinant Mouse IL-17F Protein Summary

• Description: Recombinant bioactive protein containing 266 amino acids for Mouse IL-17F
  
  

  Source:E. coli
  Amino Acid Sequence:(Q7TNI7)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: IL-17F is fully bioactive when compared to standard. The ED50 as determined by the dose-dependent induction of IL-6 in NHDF is 1.94 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL17F
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 29.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized with no additives.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in sterile H2O to 0.1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Mouse IL-17F Protein

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• interleukin 17F

Background

Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activated CD4+ T cells have also been shown to produce Mouse IL-17F. Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A. Recombinant Mouse IL-17F produced in E.Coli is a non-glycosylated polypeptide comprised of two monomeric subunit each of mIL-17F. The dimer consists of 266 amino acids, with an approximate molecular weight of 29.8 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL17F