Mouse IL-17F ELISA Kit (Colorimetric)

Mouse IL-17F ELISA Kit (Colorimetric) [NBP1-92666] - Mouse IL-17F ELISA Kit [NBP1-92666] - Data for Mouse IL-17F ELISA

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum and plasma (EDTA), buffered solution
• Standard Curve Range: 62.5 - 4,000 pg/mL
• Sensitivity: 10 pg/mL

Mouse IL-17F ELISA Kit (Colorimetric) Summary

• Description: The mouse IL-17F ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-17F.
• Specificity: The assay detects both natural and recombinant mouse IL-17F. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-17F positive sample. No crossreactivity to the IL-17AA homodimer is observed and less than 2.5% crossreactivity to IL-17AF heterodimer when spiked in excess (100ng/ml).
• Standard Curve Range: 62.5 - 4,000 pg/mL
• Sensitivity: 10 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.90%
• Intra-Assay: CV% < 4.20%
• Spike Recovery: Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL17F

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-17F ELISA Kit (Colorimetric)

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• interleukin 17F

Background

IL-17F and 17A are closely related members of the IL-17 cytokine family, sharing about 50% amino acid identity. Expression of IL-17F and IL-17A has been detected in activated human peripheral blood lymphocytes. It has been shown that the cytokines are expressed in activated human CD4+ T cells. Expression of IL-17F and IL-17A has also been observed in tissue samples from various autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma. The crystal structure of IL-17F has been solved and shows that the protein forms a disulfide-linked dimeric glycoprotein. IL-17A is also a disulfide-linked homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found in the TGF-beta, bone morphogenetic protein, and nerve growth factor superfamilies. One difference in the cysteine knot motif of IL-17F compared with the other known cysteine knot protein families is that it only utilizes four cysteines instead of the classical six cysteines to form the knot. IL-17F and IL-17A have been shown to form biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F and IL-17A homodimers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-17F ELISA Kit (NBP1-92666). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL17F