IL-17F Antibody (1058A) [Phycoerythrin]

Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rabbit Anti-Mouse IL-17F PE-conjugated Monoclonal Antibody (Catalog # IC20571P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC105P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

IL-17F Antibody (1058A) [Phycoerythrin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant mouse IL-17F
  Ala21-Val153
  Accession # Q7TNI7
• Specificity: Detects mouse IL-17F in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Il17f
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17F Antibody (1058A) [Phycoerythrin]

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• interleukin 17F

Background

IL-17F, also called ML-1, is a 21-33 kDa member of the IL-17 family of protein. It is one of six glycoproteins with conserved cysteines that form a cystine knot structure.  IL-17F is expressed by multiple cell types, including bronchial epthelium, neutrophils (likely), monocytes, mast cells, colonic epithelium, gamma δ T cells, CD4⁺ Th17 cells, group 3 (NKp46^- ROR gamma t) innate lymphoid cells, NKT cells and CD8⁺ Tc17 cells. IL-17F is known to form disulfide-linked homodimers, and covalent heterodimes with IL-17A. Notable, the IL-17A and IL-17F homodimers, plus the IL-17A:F heterodimer use the same receptor combination (IL-17RA:IL-17RC), but elicit somewhat different outcomes. For instance, on macrophages, IL-17A induces IL-9, KC and GM-CSF secretion, while IL-17F does not. Both molecules, however, induce the secretion of the same molecules (IL-9, KC, and GM-CSF) by colonic epithelium. Mouse IL-17F has a long form and a short form, which have alternate start sites but identical lengths. Over amino acids (aa) 21-153, mouse IL-17F shares 88% and 56% aa sequence identity with rat and human IL-17F, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Il17f
• Uniprot:
  • Q7TNI7()