IL-17E/IL-25 Antibody (207710)

• Reactivity: Mu
• Applications: WB
• Clone: 207710
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17E/IL-25 Antibody (207710) Summary

• Immunogen: E. coli-derived recombinant mouse IL‑17E/IL-25
  Val17-Ala169
  Accession # NP_542767
• Specificity: Detects mouse IL-17E/IL-25 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) IL‑17C, rhIL-17D, rhIL-17E, rhIL-17F, recombinant mouse (rm) IL-17, or rmIL-17B.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL25
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17E/IL-25 Antibody (207710)

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Mouse IL-17E cDNA encodes a 169 amino acid (aa) residues precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
5. Pan, G. et al. (2001) J. Immunol. 167:6569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17E/IL-25 Antibody (MAB1399)(3)

Publications using MAB1399

• Upendra Chalise, Mediha Becirovic-Agic, Jocelyn R. Rodriguez-Paar, Shelby R. Konfrst, Sharon D. B. de Morais, Catherine S. Johnson et al. Harnessing the Plasma Proteome to Mirror Current and Predict Future Cardiac Remodeling After Myocardial Infarction Journal of Cardiovascular Translational Research 2/1/2023 [PMID: 36197585]
• Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Korner H, Cua D, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide D, Matsumoto K, Nakae S Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, Is crucial for murine asthma. J Immunol, 2012-08-31;189(7):3641-52. 2012-08-31 [PMID: 22942422] (IHC-P, Mouse)
• Tamachi T, Maezawa Y, Ikeda K, Kagami S, Hatano M, Seto Y, Suto A, Suzuki K, Watanabe N, Saito Y, Tokuhisa T, Iwamoto I, Nakajima H IL-25 enhances allergic airway inflammation by amplifying a TH2 cell-dependent pathway in mice. J. Allergy Clin. Immunol., 2006-06-21;118(3):606-14. 2006-06-21 [PMID: 16950278] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IL25
• Uniprot:
  • NP_542767()