IL-17E/IL-25 Antibody (182203) [Phycoerythrin]

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human IL-17E/IL-25 PE-conjugated Monoclonal Antibody (Catalog # IC1258P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 182203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-17E/IL-25 Antibody (182203) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human IL-17E
  Tyr33-Gly177
  Accession # Q9H293
• Specificity: Detects human IL-17E in direct ELISAs and Western blots. Shows 100% cross-reactivity with recombinant mouse IL‑17E and no cross-reactivity with recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, or rhIL-17F.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL25
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17E/IL-25 Antibody (182203) [Phycoerythrin]

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, and IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17E cDNA encodes a 177 amino acid (aa) residues precursor protein with a putative 32 aa signal peptide (3). A second isoform of human IL-17E encoding a 161 aa precursor protein also exists (4). The two isoforms differ in their signal peptide sequences. Mature human IL-17E shares 76% aa sequence identity with mature mouse IL-17E. Human IL-17E also shares from 25-36% aa sequence identity with the other human IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland, spinal cord, and trachea (3). IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3), which is expressed in kidney and liver, and at lower levels in brain, testis, and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the pro-inflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
5. Pan, G. et al. (2001) J. Immunol. 167:6569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17E/IL-25 Antibody (IC1258P)(5)

Publications using IC1258P

• Yuan Zhang, Ying Wang, Ming-Qing Li, Jie Duan, Deng-Xuan Fan, Li-Ping Jin IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual ??T cells in early pregnancy. Experimental and Therapeutic Medicine, 2017-12-15;0(0):1792-0981. 2017-12-15 [PMID: 29434775] (Flow Cytometry, Human)
• Yuan Zhang, Ying Wang, Xiao‑Hui Wang, Wen‑Jie Zhou, Li‑Ping Jin, Ming‑Qing Li Crosstalk between human endometrial stromal cells and decidual NK cells promotes decidualization in�vitro by upregulating IL‑25 Molecular Medicine Reports 2017-12-12 [PMID: 29257317] (Flow Cytometry, Human)
• Wei-Bei Wang, Men-Luh Yen, Ko-Jiunn Liu, Pei-Ju Hsu, Ming-Hong Lin, Pei-Min Chen et al. Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses Stem Cell Reports 2015-09-08 [PMID: 26321145]
• Gironi , Maira, Saresella , Marina, Rovaris , Marco, Vaghi , Matilde, Nemni , Raffaell, Clerici , Mario, Grossi , Enzo A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Immun Ageing, 2013-01-10;10(1):1. 2013-01-10 [PMID: 23305498] (Flow Cytometry, Human)
• Hvid M, Vestergaard C, Kemp K IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction? J. Invest. Dermatol., 2010-09-23;131(1):150-7. 2010-09-23 [PMID: 20861853] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL25
• Uniprot:
  • Q9H293()