IL-17B Antibody (174113)

Images

 
K562 human chronic myelogenous leukemia cell line was stained with Human IL‑17B Monoclonal Antibody (Catalog # MAB1248, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, ELISA(Cap), Flow
Clone
174113
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

IL-17B Antibody (174113) Summary

Immunogen
E. coli-derived recombinant human IL-17B
Gln21-Phe180
Accession # Q9UHF5
Specificity
Detects human IL-17B in Western blots and ELISAs. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL‑17B is observed and no cross-reactivity with recombinant human (rh) IL‑17, rhIL-17C, rhIL-17E or rhIL-17F is observed. In sandwich immunoassays, less than 0.5% cross-reactivity with rmIL-17B, and no cross-reactivity with rhIL-17C, rhIL-17D, rhIL-17E, rhIL-17F, or rhIL-17 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IL17B
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human IL-17B Antibody (Catalog # MAB1248)
ELISA Detection: Human IL-17B Biotinylated Antibody (Catalog # BAM12481)
Standard: Recombinant Human IL-17B (Catalog # 1248-IB)
Publications
Read Publication using
MAB1248 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17B Antibody (174113)

  • Cytokine Zcyto7
  • cytokine-like protein ZCYTO7
  • IL17B
  • IL-17B
  • IL-17Binterleukin-17 beta
  • IL20
  • IL-20interleukin-17B
  • interleukin 17B
  • interleukin 20
  • Interleukin-20
  • MGC138900
  • MGC138901
  • neuronal interleukin-17 related factor
  • Neuronal interleukin-17-related factor
  • NIRF
  • ZCYTO7interleukin-20

Background

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro‑inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL‑17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1‑4). Human IL-17B cDNA encodes a 180 aa protein with a putative 20 aa signal peptide (5, 6). Human and mouse IL-17B share 88% amino acid sequence identity. Among IL-17 family members, IL-17B is most closely related to IL-17D, sharing 27% aa sequence homology. IL-17B is expressed highly in spinal cord, and at lower levels in brain, kidney, lung, small intestine, prostate, testes, pancreas, adrenal gland and trachea (5‑7). Expression of IL-17B has also been detected in chondrocytes in articular cartilage (2). IL-17B binds the IL-17B receptor but not IL-17 R and exhibits bioactivities distinct from those of IL-17 (5, 6).

  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  5. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
  6. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
  7. Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-17B Antibody (MAB1248)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol IL17B
Uniprot