Recombinant Human IL-15R alpha hIgG-His Protein

SDS-Page: Recombinant Human IL-15R alpha Protein [NBP2-61174] - 15 % SDS-PAGE (3ug)

Cell Proliferation: Recombinant Human IL-15R alpha Protein [NBP2-61174] - Human IL-15 R alpha inhibits Human IL-15 induced cell proliferation in the CTLL2 mouse cytotoxic T cells. The ED50 range is less or equal to 2 ng/ml.

Product Discontinued

Recombinant Human IL-15R alpha hIgG-His Protein Summary

• Description: A bioactive recombinant protein with a C-Terminal hIgG-His-tag and corresponding to the amino acids 31-205 of Human IL-15 R alpha
  
  

  Source:Baculovirus

  Amino Acid Sequence: ADPITCPPPM SVEHADIWVK SYSLYSRERY ICNSGFKRKA GTSSLTECVL NKATNVAHWT TPSLKCIRDP ALVHQRPAPP STVTTAGVTP QPESLSPSGK EPAASSPSSN NTAATTAAIV PGSQLMPSKS PSTGTTEISS HESSHGTPSQ TTAKNWELTA SASHQPPGVY PQGHSDTTLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH

• Details of Functionality: Human IL-15R alpha inhibits Human IL-15 induced cell proliferation in the CTLL2 mouse cytotoxic T cells. The ED50 range is less than or equal to 2 ng/ml
• Source: Baculovirus
• Protein/Peptide Type: Biologically Active Protein
• Gene: IL15RA
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Cell Proliferation
  • SDS-Page
• Theoretical MW: 45.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human IL-15R alpha hIgG-His Protein

• CD215 antigen
• CD215
• IL15 R alpha
• IL-15 R alpha
• IL-15 receptor subunit alpha
• IL-15R alpha
• IL15RA
• IL-15Ra
• IL-15R-alpha
• interleukin 15 receptor, alpha
• interleukin-15 receptor subunit alpha
• MGC104179

Background

IL15RA is a gene that codes for a protein that is a high-affinity receptor for interleukin-15, and can signal in both cis and trans to neighboring IL2RG-expressing cells, but the expression of different isoforms may interfere with this signal transduction, as isoforms 5-8 do not bind to IL15. The isoforms are all highly expressed in the brain, with isoform 1 being the longest with a 267 amino acid chain and a weight of approximately 28 kDa. Current studies are being done on several diseases and disorders relating to this gene including lymphoma, systemic lupus erythematosus, anemia, inflammatory bowel disease, leukemia, bronchiolitis, rheumatoid arthritis, hepatitis B, gingivitis, lung cancer and Alzheimer's disease. IL15Ra has also been shown to have interactions with SYK, IL2RB, TRAF2, and AXL in pathways such as the IL-15 signlaing, TCR signaling, and IgA production pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Bioinformatics

• Gene Symbol: IL15RA