IL-15R alpha Antibody (151303) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PHA overnight were stained with Mouse Anti-Human IL-15 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1471A) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

IL-15R alpha Antibody (151303) [Allophycocyanin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑15 R alpha
  Ile31-Thr172
  Accession # EAW86418
• Specificity: Detects human IL‑15 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha , rhIL‑2 R beta , rhIL‑2 R gamma , or rmIL‑15 R alpha is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL15RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-15R alpha Antibody (151303) [Allophycocyanin]

• CD215 antigen
• CD215
• IL15 R alpha
• IL-15 R alpha
• IL-15 receptor subunit alpha
• IL-15R alpha
• IL15RA
• IL-15Ra
• IL-15R-alpha
• interleukin 15 receptor, alpha
• interleukin-15 receptor subunit alpha
• MGC104179

Background

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha , IL-2 R beta , and IL-2 R gamma _c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-15R alpha Antibody (FAB1471A)(1)

Publication using FAB1471A

• AM Farhat, AC Weiner, C Posner, ZS Kim, B Orcutt-Jah, SM Carlson, AS Meyer Modeling cell-specific dynamics and regulation of the common gamma chain cytokines Cell Reports, 2021-04-27;35(4):109044. 2021-04-27 [PMID: 33910015] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL15RA
• Uniprot:
  • EAW86418()