Recombinant Mouse IL-13 Protein

SDS-Page: Mouse IL-13 Protein [NBP2-35103]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse IL-13 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 111 amino acids corresponding to IL-13 Source: E. coli

  Uniprot ID: P20109

  Amino Acid Sequence: MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F

• Details of Functionality: IL-13 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of > 2.5 x 10^5 IU/mg
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL13
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-13 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 12.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse IL-13 Protein

• ALRHMGC116789
• BHR1interleukin-13
• IL13
• IL-13
• IL-13MGC116788
• interleukin 13
• MGC116786
• NC30
• P600

Background

Murine Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 11 and secreted by many cell types, especially T helper type 2 (Th2) cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 has been implicated as a key factor in asthma, allergy, atopy and inflammatory response, establishing the protein as a valuable therapeutic target. Human, mouse and rat IL-3 share low homology, but have cross species activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for IL-13 Recombinant Protein (NBP2-35103)(1)

Publication using NBP2-35103

• Ai-Mei Zhang, Qian Shen, Min Li et al. Comparative studies of macrophage-biased responses in mice to infection with Toxoplasma gondii ToxoDB #9 strains of different virulence isolated from China. Parasit Vectors 2013-01-01 [PMID: 24499603]

Bioinformatics

• Gene Symbol: IL13
• Uniprot:
  • P20109()
  • P20109()
  • P20109()