IL-13 Antibody [Unconjugated]

Images

 
Recombinant Human IL‑13 (Catalog # 213-ILB) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑13 (10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

IL-13 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-13
Gly21-Asn132
Accession # AAK53823
Specificity
Detects human IL-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross‑reactivity with recombinant rhesus monkey IL-13 is observed, approximately 25% cross‑reactivity with recombinant canine IL-13 is observed, and approximately 15% cross‑reactivity with recombinant rat IL-13 is observed and less than 5% cross‑reactivity with recombinant mouse IL-13 and recombinant cotton rat IL-13 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL13
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 5 ug/10^6 cells
  • Neutralization 0.5-3 ug/mL
Publications
Read Publications using
AF-213-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13 Antibody [Unconjugated]

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus macaque IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
  4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-25241
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NB600-922
Species: Ch, V-Vi
Applications: WB, ELISA, IHC, IHC-P, IP
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF152
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
7954-GM/CF
Species: Hu
409-ML/CF
Species: Mu
NB7343
Species: Ca
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for IL-13 Antibody (AF-213-NA)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, IHC-P, Neutralization.


Filter By Application
ICC
(1)
IHC-P
(3)
Neutralization
(1)
All Applications
Filter By Species
Human
(5)
All Species

Reviews for IL-13 Antibody (AF-213-NA) (0)

There are no reviews for IL-13 Antibody (AF-213-NA). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-13 Antibody (AF-213-NA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF213

Additional IL-13 Products

Bioinformatics Tool for IL-13 Antibody (AF-213-NA)

Discover related pathways, diseases and genes to IL-13 Antibody (AF-213-NA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-13 Antibody (AF-213-NA)

Discover more about diseases related to IL-13 Antibody (AF-213-NA).
 

Pathways for IL-13 Antibody (AF-213-NA)

View related products by pathway.

PTMs for IL-13 Antibody (AF-213-NA)

Learn more about PTMs related to IL-13 Antibody (AF-213-NA).

Blogs on IL-13.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

FLICE, FLICE, baby
Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

IL-13 [HRP]
M1300CB

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-13 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol IL13
Uniprot