IL-13 Antibody (38213) [Unconjugated]

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Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
38213
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

IL-13 Antibody (38213) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL-13
Accession # P20109
Specificity
Detects mouse IL-13 in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human IL‑13 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL13
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.3-0.8 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Mouse IL-13 Biotinylated Antibody (Catalog number BAF413)
Standard: Recombinant Mouse IL-13 (Catalog number 413-ML)
Publications
Read Publications using
MAB413 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13 Antibody (38213) [Unconjugated]

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. Brown, K.D. et al. (1989) J. Immunol. 142:679.
  4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-13 Antibody (MAB413)(12)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ELISA Development, ELISA Development (Capture), Neutralization.


Filter By Application
ELISA Development
(6)
ELISA Development (Capture)
(3)
Neutralization
(3)
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Filter By Species
Mouse
(12)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB413 Applications Species
J Zhao, Y Okamoto, Y Asano, K Ishimaru, S Aki, K Yoshioka, N Takuwa, T Wada, Y Inagaki, C Takahashi, T Nishiuchi, Y Takuwa Sphingosine-1-phosphate receptor-2 facilitates pulmonary fibrosis through potentiating IL-13 pathway in macrophages PLoS ONE, 2018;13(5):e0197604. 2018 [PMID: 29782549] (Neutralization, Mouse) Neutralization Mouse
SR Damle, RK Martin, JV Cross, DH Conrad Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis Mucosal Immunol, 2016;0(0):. 2016 [PMID: 27049059] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Knudson C, Hartwig S, Meyerholz D, Varga S RSV vaccine-enhanced disease is orchestrated by the combined actions of distinct CD4 T cell subsets. PLoS Pathog, 2015;11(3):e1004757. 2015 [PMID: 25769044] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome. Mucosal Immunol, 2014;7(2):417-27. 2014 [PMID: 24022789] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Mosconi E, Rekima A, Seitz-Polski B Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development. Mucosal Immunol, 2010;3(5):461-74. 2010 [PMID: 20485331] (ELISA Development, Mouse) ELISA Development Mouse
Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease. J. Immunol., 2010;184(8):4159-69. 2010 [PMID: 20228193] (ELISA Development, Mouse) ELISA Development Mouse
Neveu WA, Allard JB, Dienz O, Wargo MJ, Ciliberto G, Whittaker LA, Rincon M IL-6 is required for airway mucus production induced by inhaled fungal allergens. J. Immunol., 2009;183(3):1732-8. 2009 [PMID: 19592651] (ELISA Development, Mouse) ELISA Development Mouse
Daniel C, Sartory NA, Zahn N FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions. Mol. Immunol., 2007;44(13):3305-16. 2007 [PMID: 17475330] (Neutralization, Mouse) Neutralization Mouse
Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia. Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(5):L862-70. 2006 [PMID: 16782750] (ELISA Development, Mouse) ELISA Development Mouse
Wen H, Hogaboam CM, Gauldie J, Kunkel SL Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006;168(6):1940-50. 2006 [PMID: 16723709] (ELISA Development, Mouse) ELISA Development Mouse
Show All 12 Publications.

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Bioinformatics

Gene Symbol IL13
Uniprot