Recombinant Rat IL-12 Protein

Product Discontinued

Recombinant Rat IL-12 Protein Summary

• Description: Recombinant bioactive protein containing 503 amino acids for Rat IL-12
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NP_072133)

• Details of Functionality: The ED50 range is 0.01-0.05 ng/ml, as determined by the dose-dependent secretion of IFN-g from PHA and IL-12 activated mouse splenocytes that has been co-stimulated with PMA.
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: IL12A
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from solution with no additives.
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in H2O to a concentration of 0.1 - 0.5 mg/ml. The solution can be diluted into other aqueous buffers.

Alternate Names for Recombinant Rat IL-12 Protein

• CLMF p35
• CLMF1
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL-12 subunit p35
• IL12
• IL-12
• IL-12, subunit p35
• IL12A
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

The recombinant rat IL-12 produced from Sf9 insect cells is a glycosylated disulfide linked hetromeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa and 40 kDa subunits

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL12A