IL-12 p70/IL-12A Antibody (48110)

• Reactivity: Mu
• Applications: WB, ELISA(Cap)
• Clone: 48110
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-12 p70/IL-12A Antibody (48110) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12
• Specificity: Detects mouse IL-12 in ELISAs and Western blots. Detects an epitope in the p35 subunit. In sandwich ELISAs, does not cross-react with recombinant mouse IL-12 p40.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL12A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse IL-12 Biotinylated Antibody (Catalog number BAF419)
  Standard: Recombinant Mouse IL-12 (Catalog number 419-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 p70/IL-12A Antibody (48110)

• CLMF p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL12 p70
• IL-12 p70
• IL-12 subunit p35
• IL-12, subunit p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12 p70/IL-12A Antibody (MAB419)(15)

Publications using MAB419

• Ling Li, Xiaojun Sun, Sisi Wu, Xin Yuan, Bingxin Liu, Xueping Zhou Interleukin-12 exacerbates symptoms in an MRL/MpJ-Faslpr mouse model of systemic lupus erythematosus Experimental and Therapeutic Medicine 4/15/2021 [PMID: 33936283]
• W Dawicki, C Li, J Town, X Zhang, JR Gordon Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells J. Allergy Clin. Immunol., 2016-10-26;139(5):1608-1620.e3. 2016-10-26 [PMID: 28277274] (ELISA Development (Capture), Mouse)
• Khalid Z Matalka Eriobotrya japonica Water Extract Characterization: An Inducer of Interferon-Gamma Production Mainly by the JAK-STAT Pathway Molecules, 2016-06-02;21(6):. 2016-06-02 [PMID: 27271577] (ELISA Development, Mouse)
• Badr M, Qinna N, Qadan F, Matalka K Dichloroacetate modulates cytokines toward T helper 1 function via induction of the interleukin-12-interferon-gamma pathway. Onco Targets Ther, 2014-02-07;7(0):193-201. 2014-02-07 [PMID: 24532971] (Functional Assay, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Jin W, Qi S, Luo H The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function. BMC Immunol., 2011-12-19;12(0):68. 2011-12-19 [PMID: 22182253] (Neutralization, Human)
• Divangahi M, Mostowy S, Coulombe F, Kozak R, Guillot L, Veyrier F, Kobayashi KS, Flavell RA, Gros P, Behr MA NOD2-deficient mice have impaired resistance to Mycobacterium tuberculosis infection through defective innate and adaptive immunity. J. Immunol., 2008-11-15;181(10):7157-65. 2008-11-15 [PMID: 18981137] (ICC, Mouse)
• Liu J, Dolan MC, Reidy M, Cramer CL Expression of bioactive single-chain murine IL-12 in transgenic plants. J. Interferon Cytokine Res., 2008-06-01;28(0):381-92. 2008-06-01 [PMID: 18593333] (ELISA Development, Mouse)
• Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis. J. Immunol., 2006-12-15;177(12):8650-7. 2006-12-15 [PMID: 17142765] (ELISA Development, Mouse)
• Chiang YM, Chang CL, Chang SL, Yang WC, Shyur LF Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator. J Ethnopharmacol, 2006-10-19;110(3):532-8. 2006-10-19 [PMID: 17101254] (Neutralization, Mouse)
• Wen H, Hogaboam CM, Gauldie J, Kunkel SL Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006-06-01;168(6):1940-50. 2006-06-01 [PMID: 16723709] (ELISA Development, Mouse)
• An HJ, Jeong HJ, Um JY, Kim HM, Hong SH Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. J Ethnopharmacol, 2006-03-10;106(3):418-24. 2006-03-10 [PMID: 16530364] (ELISA Development, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA Development, Mouse)
• Rosini L, Matlack R, Taylor J, Howell KF, Yeh K, Pennello A, Riggs JE Nonlymphoid peritoneal cells suppress the T cell response to Mls. Immunobiology, 2004-01-01;209(8):575-84. 2004-01-01 [PMID: 15638126] (Neutralization, Mouse)
• Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood, 2004-05-13;104(5):1404-10. 2004-05-13 [PMID: 15142882] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL12A