IL-12/IL-35 p35 Antibody (B-T21) - Azide and BSA Free

ELISA: IL-12/IL-35 p35 Antibody (B-T21) [NBP3-14604] - ELISA quantification of IFNg secretion by PBMC (PHA activated after 72 hours) with IL-12/IL-35 antibody (300 pg/ml) and different concentration of B-T21 or isotype control.

• Reactivity: Hu
• Applications: ELISA, Func
• Clone: B-T21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

IL-12/IL-35 p35 Antibody (B-T21) - Azide and BSA Free Summary

• Description: Stable at 4C for 12 months.
  
  Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells
• Immunogen: Recombinant human IL-12/IL-35 p35
• Specificity: recognizes both natural and recombinant human IL-12/IL-35 p35
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Functional
• Application Notes: Biological Activity: Inhibits IL-12 induced IFNg synthesis on PHA activated lymphocytes

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS. Sterile-filtered through 0.22 um.
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for IL-12/IL-35 p35 Antibody (B-T21) - Azide and BSA Free

• CLMF p35
• CLMF
• cytotoxic lymphocyte maturation factor 1, p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL-12, subunit p35
• IL-12/IL-35 p35
• IL-12A
• IL35 subunit
• IL35p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• NF cell stimulatory factor chain 1
• NFSK
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL12A