IL-12/IL-35 p35 Antibody (45806)

IL-12/IL-35 p35 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse IL-12/IL-35 p35 Monoclonal Antibody (Catalog # MAB6688) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 45806
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-12/IL-35 p35 Antibody (45806) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived mouse IL-12/IL-35 p35
  Arg23-Ala215
  Accession # NP_032377
• Specificity: Detects mouse IL-12/IL-35 p35 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) IL-12 p35+p40 heterodimer or recombinant rat IL-12 p35 is observed. No cross-reactivity with recombinant human IL-12 p35, rmIL-12 p40, rmIL-23 p19+p40 heterodimer, recombinant canine IL-12 p35+p40 heterodimer, or recombinant feline IL-12 p35+p40 heterodimer is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Il12a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-35 p35 Antibody (45806)

• CLMF p35
• CLMF
• cytotoxic lymphocyte maturation factor 1, p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL-12, subunit p35
• IL-12/IL-35 p35
• IL-12A
• IL35 subunit
• IL35p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• NF cell stimulatory factor chain 1
• NFSK
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-35 p35 Antibody (MAB6688)(6)

Publications using MAB6688

• Shengsong Chen, Jingen Xia, Yi Zhang, Qingyuan Zhan IL35 attenuated LPS-induced acute lung injury by regulating macrophage polarization Molecular Biology Reports 7/1/2022 [PMID: 35748972]
• X Li, W Cui, L Hull, L Wang, T Yu, M Xiao IL-18 binding protein (IL-18BP) as a novel radiation countermeasure after radiation exposure in mice Sci Rep, 2020-10-29;10(1):18674. 10/29/2020 [PMID: 33122671]
• RO Bello, MA Abdullah, R Abd Majid, VK Chin, MF Abd Rachma, ZO Ibraheem, MK Hussain, MG Magaji, R Basir IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice Malar. J., 2019-12-19;18(1):434. 2019-12-19 [PMID: 31856836] (IHC-P, Mouse)
• J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice EBioMedicine, 2018-09-15;35(0):29-39. 2018-09-15 [PMID: 30228093] (IHC, Human)
• X Yang, X Yu, Y Wei Lentiviral delivery of novel fusion protein IL12/FasTI for cancer immune/gene therapy PLoS ONE, 2018-07-25;13(7):e0201100. 2018-07-25 [PMID: 30044833] (ICC, Human)
• Bettini M, Castellaw AH, Lennon GP, Burton AR, Vignali DA Prevention of autoimmune diabetes by ectopic pancreatic beta-cell expression of interleukin-35. Diabetes, 2012-03-16;61(6):1519-26. 2012-03-16 [PMID: 22427377] (IHC, Mouse)

Bioinformatics

• Gene Symbol: Il12a
• Uniprot:
  • NP_032377()