IL-12/IL-35 p35 Antibody (45806) [Janelia Fluor® 549]

• Reactivity: Mu
• Applications: IHC
• Clone: 45806
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 549

IL-12/IL-35 p35 Antibody (45806) [Janelia Fluor® 549] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived mouse IL-12/IL-35 p35
  Arg23-Ala215
  Accession # NP_032377
• Specificity: Detects mouse IL-12/IL-35 p35 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) IL-12 p35+p40 heterodimer or recombinant rat IL-12 p35 is observed. No cross-reactivity with recombinant human IL-12 p35, rmIL-12 p40, rmIL-23 p19+p40 heterodimer, recombinant canine IL-12 p35+p40 heterodimer, or recombinant feline IL-12 p35+p40 heterodimer is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Il12a
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IL-12/IL-35 p35 Antibody (45806) [Janelia Fluor® 549]

• CLMF p35
• CLMF
• cytotoxic lymphocyte maturation factor 1, p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL-12, subunit p35
• IL-12/IL-35 p35
• IL-12A
• IL35 subunit
• IL35p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• NF cell stimulatory factor chain 1
• NFSK
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Il12a