Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)

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ELISA: Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric) [NBP3-06741] - Samples were spiked with high concentrations of Rat IL-12/IL-23 p40 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric) [NBP3-06741] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
12.5 - 800 pg/mL
Sensitivity
7.5 pg/mL

Order Details

Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat IL-Y (IL-12p40/IL-27p28) in samples. No significant cross-reactivity or interference between Rat IL-Y (IL-12p40/IL-27p28) and analogues was observed.
Standard Curve Range
12.5 - 800 pg/mL
Sensitivity
7.5 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.8
Intra-Assay
4.72
Spike Recovery
85 - 102 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL12B

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)

  • CLMF p40
  • CLMF
  • CLMF2
  • Cytotoxic lymphocyte maturation factor 40 kDa subunit
  • IL12 p40
  • IL-12 p40
  • IL-12 subunit p40
  • IL12B
  • IL-12B
  • IL-12BNK cell stimulatory factor chain 2
  • interleukin 12, p40
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
  • interleukin-12 beta chain
  • interleukin-12 subunit beta
  • natural killer cell stimulatory factor, 40 kD subunit
  • NKSF
  • NKSF2
  • NKSF2IL12, subunit p40

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for IL-12/IL-23 p40 ELISA Kit (NBP3-06741). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol IL12B