IL-11 Antibody [Unconjugated]

Recombinant Human IL‑11 (Catalog # 218-IL) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-218-NA). The ND50 is typically 0.06‑0.48 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-11 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑11
  Pro22-Leu199
  Accession # P20809
• Specificity: Detects human IL-11 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL11
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.06-0.48 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-11 Antibody [Unconjugated]

• Adipogenesis inhibitory factor
• AGIF
• AGIFoprelvekin
• IL11
• IL-11
• IL-11Oprelvekin
• interleukin 11
• interleukin-11
• Oprelvekin

Background

Interleukin 11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The human IL-11 cDNA encodes a 199 amino acid residue precursor polypeptide with a 21 amino acid residue hydrophobic signal that is processed proteolytically to generate the 178 amino acid residue mature protein. IL-11 contains no cysteine residues or potential glycosylation sites.

IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G₀ period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell. IL-11, in the presence of IL-3 or SCF, has also been shown to stimulate erythropoiesis. Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute-phase proteins. Consistent with the in vitro functions of IL‑11, in vivo administration of rhIL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors.

IL-11 exerts its biological activities through binding to a specific high-affinity receptor having an apparent molecular mass of 150 kDa. Although the IL-11 binding subunit of the receptor complex has not yet been cloned, evidence suggests that, similar to IL-6, leukemia inhibitory factor, oncostatin M, and ciliary neurotrophic factor, IL-11 utilizes the IL-6 signal transducer, gp130, for signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-11 Antibody (AF-218-NA)(4)

Publications using AF-218-NA

• Maria Myzithras, Siqi Lin, Legairre Radden, Cynthia Hess Kenny, Zheng Cai, Angus MacDonald et al. Development of novel ultra-sensitive IL-11 target engagement assays to support mechanistic PK/PD modeling for an anti-IL-11 antibody therapeutic mAbs 12/31/2022 [PMID: 35916739]
• Wei-Luo Cai, Wen-Ding Huang, Bo Li, Tian-Rui Chen, Zhen-Xi Li, Cheng-Long Zhao et al. microRNA-124 inhibits bone metastasis of breast cancer by repressing Interleukin-11 Molecular Cancer 12/1/2018 [PMID: 29343249]
• Andersson By U, Tani E, Andersson U Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis. J. Pediatr. Hematol. Oncol., 2004-11-01;26(11):706-11. 2004-11-01 [PMID: 15543003] (ICC, Human)
• Dimitriadis E, Robb L, Salamonsen LA Interleukin 11 advances progesterone-induced decidualization of human endometrial stromal cells. Mol. Hum. Reprod., 2002-07-01;8(7):636-43. 2002-07-01 [PMID: 12087078] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL11
• Uniprot:
  • P20809()